Thermo Orbitrap Fusion

The Thermo Scientific Orbitrap Fusion Tribrid mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers unprecedented depth of analysis. It enables life scientists analyzing even the most challenging low-abundance, high-complexity, or difficult samples to identify more compounds more quickly, quantify
more accurately, and elucidate structures more thoroughly.
 

The Orbitrap Fusion system’’s next-generation hardware and software make setup easier, methods more powerful, and operation more robust. Users can spend more time thinking about their research and less time worrying about method development and experimental execution.

Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion Tribrid mass spectrometer delivers unmatched analytical performance with revolutionary depth of analysis, unprecedented usability, and exceptional robustness.
 

• Resolution up to 450,000 to remove spectral interferences
• CID/HCD/ETD detected by the Ion Trap or Orbitrap at any level of MSn for maximum experimental flexibility
• Dynamic Scan Management architecture lets every user easily get the maximum information from every sample

Mass Range

Linear Ion Trap and Orbitrap Mass Range:

Standard  m/z 50 - 2,000
High Mass  m/z 200 - 4,000

Orbitrap Extended Mass Range:

Up to m/z 6000 without precursor ion selection

 

Resolution

15,000-450,000 (FWHM) at m/z 200
 

Scan Rate

Orbitrap MSn up to 20 Hz
Ion trap MSn up to 20 Hz
 

Mass Accuracy

< 3 ppm RMS using external calibration
< 1 ppm RMS using internal calibration
 

MS/MS Sensitivity of Ion Trap

2 μL of a 50 fg/μL solution of reserpine (100 fg total) injected at a flow of 500 μL/min will produce a minimum signal-to-noise ratio of 100:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, m/z 165 - 615. The test requires the HESI probe.

Dynamic Range

> 5,000 within a single scan, guaranteeing specified mass accuracy
 

MS Scan Power

MSn, for n = 1 through 10
 

Thermo Scientific ’’EASY-ETD’’ Ion Source

- Generates fluoranthene anions for electron transfer dissociation (ETD)
- Townsend discharge ionization is extremely stable and robust
- Compact size, located entirely within the footprint of the instrument
- Active reagent ion filtering using mass-resolving quadrupole

Thermo Scientific ’’EASY-IC’’ Ion Source

- Generates internal calibrant ions for real-time mass calibration on every spectrum
- Provides <1 ppm RMS mass accuracy

Synchronous Precursor Selection

Up to 15 precursors per MS² scan, for MS³ analysis only
 

Multiplexing Using Ion-Routing Multipole

Up to 10 precursors per scan using quadrupole mass filter
 

Polarity Switching

One full cycle in 1.1 sec (one full scan in positive mode and one full scan in negative mode at resolution setting of 35,000)