Shimadzu Axima TOF2

The AXIMA-TOF2 is one of the most powerful tools in mass spectrometry, delivering information-rich spectra with greater sensitivity and higher confidence in identification. It is an extremely versatile and powerful TOF-TOF system, integrating workflows for a diverse range of analytical needs. This system can analyse and process proteins, polymers, oligonucleotides, SNPs, metabolites, lipids, carbohydrates and small molecules. The AXIMA-TOF2 is designed for a wide range of applications like LC-MALDI based experiments, the recognition of biomarker patterns and functional genomics applications.

Key benefits and features

• Delivers the highest energy collisions (CID with laboratory energy of 20keV) to unlock more information with higher sensitivity.
• Incorporates novel ion gate technology for high-resolution precursor ion selection, simplifying TOF-TOF spectra and data interpretation, even in the presence of complex mixtures.
• Opens new horizons in analytical research from peptide characterisation, protein biomarker discovery and lipidomics to polymer characterisation.
• Helps to increase sample throughput and data quality with intelligent results-dependent analysis.

Mass Range
Linear - 1 to 500kDA
Reflectron - 1 to 80 kDa

Mass Resolution
Linear - >5000 FWHM - ACTH 18-39((M+H))+ 2465 Da
Reflectron - >20,000 FWHM - ACTH 7-38 ((M+H)+ 3660 Da)
MS/MS - isotopic resolution of fragments - Angiotenisn II

Accuracy
Linear
<30 ppm with internal calibration
<200 ppm with external calibration (Nearest neighbour external calibration on 384 well sample target, within 30 minutes)

Reflectron
<5 ppm with internal calibration
<50 ppm with external calibration (Nearest neighbour external calibration on 384 well sample target, within 30 minutes)

MS/MS
<150 ppm with external calibration

ion gate resolution
>400 FWHM at 1000 Da

Sensitivity
Linear
250 fmol - bovine serum albumin (loaded)
250 amol - Glu-1-Fibrinopeptide B (loaded)

Reflecton
250 amol - Glu-1-Fibrinopeptide B (loaded)

MSMS
2.5 fmol - Glu-1-Fibrinopeptide B (loaded)

All specifications are run on a standard 2 mm, 384 well, stainless steel sample plate unless otherwise stated.